by Expert Design for High-Affinity Custom Polyclonal Antibodies
Our 25+ years of expertise in antibody design and manufacturing will make sure you one of the best likelihood of getting a excessive affinity polyclonal antibody that works to your utility. Our crew of immunology specialists can be with you each step of the way in which.
Antigen design and expression overview
Design
We will design the antigen utilizing our a long time of expertise in immunogen design, taking consideration of a wide range of components together with sequence homology, post-translational modifcation, secondary/tertiary construction and epitope localization.
Expression
We recombinantly specific the antigen with our proprietary protein folding method to make sure greatest tertiary construction accuracy of the antigen to the goal protein’s pure type.
User Provided Antigen
If you resolve to supply your individual recombinant protein or peptide antigen, we’d like greater than Eight mg of antigen with 80%+ purity, at above 1mg/ml focus.
Immunization & Purification
4mg of antigen can be utilized in immunization and 4mg of antigen can be utilized in affinity purification. If you solely have 4mg of antigen, we are able to accommodate through the use of protein A and protein G for purification, upon your request.
Immunization schedule overview
Bosterbio’s commonplace immunization protocol for polyclonal antibody manufacturing is optimized to provide high-afnity antibodies. We might regulate the protocol to provide the antibody quicker upon your particular request, waiving our afnity assure.
The protocol makes use of two New Zealand White rabbits which are Specifc-Pathogen-Free. Immunization injections use Complete Freund’s Adjuvant (CFA) or Incomplete Freund’s Adjuvant (IFA). The following desk reveals the usual protocol and deliverables.
Deliverables
- Pre-immune serum: if ordered, pre -immune serum can be delivered earlier than the start of the undertaking.
- Test-bleed at 2nd immunization booster (third injection): we’ll afnity purify the bleed and take a look at the antibody for immune response for the antigen. The report can be included within the fnal antibody manufacturing report. Upon particular request we are able to additionally present a vial of purifed pattern antibody from this bleed to you to check.
- Antibody purifcation and validation report: after fnishing the third immunization booster (4th injection), we’ll acquire the bleeds and take a look at the purifed antibody in direct ELISA assay towards the immunogen. The outcomes can be written right into a report and offered to you.
- Antibody purifcation and validation report: after fnishing the third immunization booster (4th injection), we’ll acquire the bleeds and take a look at the purifed antibody in direct ELISA assay towards the immunogen. The outcomes can be written right into a report and offered to you.
High Specificity Antibody
Both the take a look at bleed and the ultimate supply of antibodies are immunoaffinity purified utilizing antigen affinity chromatography. Guaranteed excessive affinity—we assure that at 1:1000 dilution ration the antibody can detect as little as 1ng of the protein antigen, or 100ng of the peptide antigen, in a direct ELISA.
Send us your inquiry to assist@bosterbio.com
and our crew will conduct a feasibility evaluation at no cost.
Project Overview
Phase 1: Antigen Synthesis
Add on Items
Add Recombinant Antigen
If you want among the antigen we generate to your undertaking.
Add Blocking Peptide
If you want among the antigen we are able to present it.
Standard Package
Antigen Design & Development
Included
Our crew will assist you to design one of the best antigen to your supposed utility. We think about a number of components uniqueness of the antigen hydrophobic/hydrophilic composition secondary construction (alpha-helical tendencies, beta-turn tendencies and all) and a wide range of different components. Based on the character of your goal utility, and the chemical nature of the antigen, we’ll design an immunogen distinctive to your objective.
- Phase 1 period: Approximately Four weeks/28 days
- Phase 2: Immunization
Add Pre-immune serum screening
Custom antibody tasks can fail as a result of animal having present immunity or immune-tolerance in direction of your antigen of curiosity. As an non-obligatory service, we are able to ship you pre-immune sera to display screen for this challenge earlier than your undertaking begins. We may carry out the screening for you.
Pre-immune serum screening
Add Rabbits
Adding extra rabbits will enhance your success price
and enhance the yield.
- Immunization
2 Rabbits Included - Immunization preparation Day 1
Preparing antigen with CFA, getting ready rabbits. - Immunization main injection Day 8
10 subcutaneous immune enhance injections, 0.5mg of antigen in CFA every - Immunization 2nd shot Day 22
Four subcutaneous immune enhance injections, 0.25mg of antigen in IFA every - Immunization third shot Day 36
Four subcutaneous immune enhance injections, 0.25mg of antigen in IFA every - Immunization 4th shot Day 51
Four subcutaneous immune enhance injections, 0.25mg of antigen in IFA every - Add Validation
Boster Bio affords a full service antibody validation CRO.
We can carry out IHC, WB, IF, ICC and Flow.
Sera assortment and ELISA take a look at Day 65
Direct ELISA Included
Testing the anti-sera by way of ELISA,
resolve to proceed or terminate undertaking
Add Application Insurance
Additional insurance coverage you should purchase: 100% assure that your
antibody will work in your utility or distinctive assay.
Serum purification & remaining supplyDay 72
Affinity Purification Included
Deliverables: ELISA take a look at report, 6mg of the purified antibody
*Shipping might take 7-10 enterprise days.
Add Conjugation
Additional service: conjugate your antibody
to a conjugate of your alternative
Final SupplyDay 80
Phase 2 period: Approximately 12 weeks/80 days
Summary
Timeline
User offered antigen: 11 weeks plus delivery
Boster Bio produces antigen: 15 weeks plus delivery
*Times are approximations. Actual timeline might fluctuate.
Included Items Standard Package $1300
Antigen Design
Antigen Production
Immunization of two Rabbits
Direct ELISA validation
Affinity Purification
Deliverables: 3-5mg antibody
Additional Items
Recombinant Antigen 1mg/180 euro
Blocking Peptide 100ug/eur150
Pre-Immune serum bleed eur100 / 1ml per rabbit
Additonal Rabbits eur200 every
Additional Validation See Antibody Validation Page
Conjugation 100ul/eur50 inquire bulk
Application Insurance 1000 euro
Custom Buffer Inquire
Guarantee
We assure that at 1:1000 dilution ratio on the antibody can detect
as little as 1ng of the protein antigen, or 100ng of the
peptide antigen, in a direct ELISA.
Minimum yield of 3mg
If failure at antigen expression, you solely pay 200 euro
If failure at direct ELISA take a look at, you solely pay 500 euro
Genprice
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